Aminated Plates

Aminated Plates are microplates that feature surfaces functionalized with primary amine (-NH₂) groups. These reactive groups enable covalent attachment of biomolecules, such as proteins, peptides, nucleic acids, and small molecules, through amide or imine bond formation. Aminated plates are widely used in applications requiring robust and stable biomolecule immobilization, particularly in bioassays, diagnostics, and drug development.

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Key Features and Characteristics

• Material and Surface Modification:
  • Typically constructed from polystyrene or glass.
  • Functionalized with a uniform layer of primary amine groups using chemical methods such as silanization or polymer coating.
• Chemical Properties:
  • The surface is positively charged at neutral pH.
  • Reacts readily with carboxyl, aldehyde, or other functional groups on biomolecules via covalent coupling.
• Surface Uniformity:
  • Provides a homogenous layer for consistent immobilization.
  • Reduces variability in assay performance.

Applications

• Biomolecule Immobilization:
  • Covalent coupling of proteins (e.g., antibodies, enzymes) for ELISA and other immunoassays.
  • Attachment of oligonucleotides for DNA/RNA hybridization assays or PCR arrays.
• Drug Screening:
  • Used in high-throughput screening for drug candidates interacting with immobilized targets.
  • Enables robust assay conditions by preventing target desorption.
• Diagnostics:
  • Development of diagnostic assays where stable and specific biomolecule binding is critical.
  • Detection of biomarkers, antigens, or antibodies.
• Cell Culture:
  • Supports attachment of cells that interact with amine-functionalized surfaces.
  • Useful in studying cell adhesion, growth, and differentiation.

Aminated Plates are essential tools in modern molecular biology, diagnostics, and drug discovery. Their ability to covalently attach biomolecules makes them ideal for applications requiring stable and reproducible results under a variety of conditions.