Antibodies Coated Plates

Antibodies Coated Plates are microplates pre-coated with specific antibodies to facilitate various immunoassays. These plates are designed to capture and quantify antigens or proteins that bind to the immobilized antibodies. They are widely used in applications such as enzyme-linked immunosorbent assays (ELISA), antibody screening, and protein detection.

• Material Composition:
  • Typically made from polystyrene or other suitable plastics that provide a stable and consistent surface for antibody immobilization.
  • The surface of the plates is modified to enhance the binding and stability of antibodies.
• Antibody Coating:
  • Plates are coated with specific antibodies through a process that ensures uniform and effective immobilization.
  • The coating process involves incubating the plate with a solution of the antibody, followed by blocking non-specific binding sites to minimize background noise.
• Binding Specificity:
  • The antibodies are selected based on their specific affinity for the target antigen or protein.
  • The immobilized antibodies retain their ability to bind to their target molecules, making the plates suitable for detecting and quantifying specific proteins or antigens.
• Plate Formats:
  • Available in standard formats such as 96-well and 384-well plates, allowing for both low- and high-throughput applications.
  • Typically flat-bottom designs are used, which are compatible with various detection methods including colorimetric, fluorescent, and chemiluminescent assays.
• Applications:
  • Enzyme-Linked Immunosorbent Assays (ELISA): Used to detect and quantify specific antigens or proteins in a sample by utilizing the antibody-coated plate to capture the target.
  • Immunoassays: Facilitates various types of assays where the binding of antibodies to specific antigens is measured.
  • Antibody Screening: Used to screen for the presence of specific antibodies in a sample, such as in clinical diagnostics or research.
  • Protein Detection: Allows for the detection and quantification of proteins based on their interaction with immobilized antibodies.
• Compatibility:
  • Compatible with a range of detection systems, including colorimetric (e.g., HRP/TMB), fluorescent, and chemiluminescent assays.
  • Suitable for use with standard laboratory equipment such as microplate readers, automated liquid handlers, and plate washers.
• Well Volume:
  • Designed to accommodate typical assay volumes, usually ranging from 50 µL to 300 µL per well, depending on the specific plate format and assay requirements.
  • Ensures consistent well-to-well volume, which is crucial for reproducibility in high-throughput experiments.