Antibodies Coated Plates

Antibody-Coated Plates are microplates pre-coated with specific antibodies on their surface. These plates are widely used in immunoassays, diagnostics, and research applications for capturing target antigens from complex samples. The immobilized antibodies enable high specificity and sensitivity in detecting analytes, making them essential tools in enzyme-linked immunosorbent assays (ELISA), immunoprecipitation, and other bioassays.

Content: 

Key Features and Characteristics

• Antibody Immobilization:
  • Antibodies are covalently or adsorptively bound to the plate surface.
  • Orientation and stability are optimized to preserve binding activity.
• Surface Chemistry:
  • Plates are typically made of polystyrene or glass.
  • Pre-coated surfaces may include passive adsorption or covalent attachment methods (e.g., amine or carboxyl coupling).
• Binding Specificity:
  • Coating involves high-affinity antibodies specific to the target analyte.
  • Reduces cross-reactivity and nonspecific binding in assays.
• Plate Formats:
  • Available in 96-well, 384-well, or other formats compatible with high-throughput systems.
  • Suitable for colorimetric, fluorescence, or chemiluminescence detection methods.

Applications

• Immunoassays (ELISA):
  • Capture and detect target antigens from biological samples such as serum, plasma, or cell lysates.
  • Supports sandwich, competitive, and indirect ELISA formats.
• Diagnostics:
  • Detection of biomarkers for diseases, infections, or conditions.
  • Used in clinical laboratories for point-of-care testing or large-scale screening.
• Protein Interaction Studies:
  • Investigate interactions between antigens, antibodies, or other proteins.
  • Used in studying immune response and characterizing epitopes.
• Drug Discovery:
  • Screening and characterization of therapeutic antibodies or antigen-binding molecules.
  • Evaluation of drug efficacy in binding or neutralizing specific targets.

Antibody-Coated Plates provide a robust and reliable platform for sensitive and specific detection of antigens. Their ease of use and compatibility with diverse applications make them indispensable in diagnostics, research, and drug development.