Biotine Coated Plates
Biotin-coated plates are specialized laboratory consumables designed for binding biotinylated molecules. These plates are commonly used in biological assays and diagnostics due to their ability to specifically interact with streptavidin, avidin, or other biotin-binding proteins. The biotin coating provides a uniform surface that ensures high binding efficiency and specificity for downstream applications.
Content and Properties of Biotin-Coated Plates
- Composition:
- The plates are typically made of polystyrene or similar materials, coated with biotin via covalent or adsorption-based methods.
- High-binding variants ensure the maximum immobilization of biotinylated molecules.
- Surface Chemistry:
- The biotin layer is stable, preventing leaching during washing or incubation steps.
- Functionalized surfaces are optimized for minimal non-specific binding.
- Physical Specifications:
- Available in multiple formats: 96-well, 384-well, and other microplate configurations.
- Compatible with automated systems and high-throughput assays.
Applications of Biotin-Coated Plates
- Protein and Peptide Immobilization:
- Used to immobilize biotinylated antibodies, antigens, or peptides for enzyme-linked immunosorbent assays (ELISA).
- Molecular Interactions Studies:
- Suitable for analyzing protein-protein, protein-DNA, or protein-ligand interactions using techniques like surface plasmon resonance (SPR) or fluorescence.
- Drug Discovery:
- Utilized in screening assays where biotinylated molecules act as capture agents for target proteins or small molecules.
- Signal Amplification:
- Enhance assay sensitivity by leveraging the high affinity between biotin and streptavidin in signal detection.
- Diagnostics:
- Key component in diagnostic kits for detecting biomarkers in clinical samples.
Biotin-coated plates are integral tools in molecular biology, immunology, and pharmaceutical research, offering robust and specific platforms for a variety of biomolecular assays.
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