Biotin Coated Plates

Biotin Coated Plates (AffiPLATE) are specialized microplates designed for high-affinity binding of streptavidin or avidin-conjugated molecules. The surface of these plates is uniformly coated with biotin, a small molecule with a strong affinity for streptavidin and avidin. These plates are typically used in various biochemical assays, including ELISA (Enzyme-Linked Immunosorbent Assay), affinity purification, and other applications that require precise binding interactions.

Plate Composition

• Plates are made from polystyrene or other plastics optimized for biochemical assays.
• Surface is covalently coated with biotin molecules.

Binding Capacity

• High biotin density ensures optimal binding sites for streptavidin/avidin.
• Typically supports consistent and reproducible binding interactions.

Applications

• ELISA: Used in sandwich or competitive ELISAs where biotin-labeled antibodies or antigens are captured by streptavidin or avidin.
• Affinity Purification: Enables the selective capture of biotinylated molecules from complex mixtures.
• Drug Screening: Facilitates high-throughput screening of biotin-labeled compounds.

Stability

• Biotin coating is stable under standard laboratory conditions.
• Plates are often stored dry and are rehydrated before use.

Compatibility

• Suitable for use with a wide range of buffers and reagents commonly used in biochemical assays.
• Compatible with standard laboratory equipment like plate readers.

Specifications

• Available in various formats (e.g., 96-well, 384-well) depending on the assay needs.
• Each well has a defined biotin coating, ensuring uniform assay conditions.

Handling and Use

• Plates should be handled with care to avoid contamination.
• Reagents should be added gently to avoid disrupting the biotin layer.