Carboxylated Plates

Carboxylated Plates are microplates functionalized with carboxylic acid (-COOH) groups on their surface. These plates are designed for covalent attachment of biomolecules, leveraging the carboxyl groups' ability to react with primary amines under appropriate coupling conditions. Carboxylated plates are widely used in immunoassays, molecular biology, drug screening, and biosensor development.

Content: 

Key Features and Characteristics

• Material and Surface Chemistry:
  • Constructed from materials such as polystyrene, glass, or other inert polymers.
  • Functionalized with a uniform layer of carboxylic acid groups through chemical modification techniques.
• Chemical Properties:
  • The negatively charged surface facilitates electrostatic interactions at neutral to basic pH.
  • Reacts with primary amines via carbodiimide chemistry (e.g., EDC/NHS coupling) to form stable amide bonds.
• Surface Homogeneity:
  • Uniform distribution of carboxyl groups ensures consistent biomolecule immobilization.
  • Reduces variability and enhances assay reproducibility.
• Compatibility:
  • Suitable for use with various detection platforms, including fluorescence, colorimetric, and electrochemical assays.

Applications

• Biomolecule Immobilization:
  • Covalent attachment of proteins (e.g., antibodies, enzymes), peptides, or nucleic acids for use in diagnostic assays or research studies.
  • Immobilization of oligonucleotides for microarray analysis or hybridization studies.
• Immunoassays:
  • Used in enzyme-linked immunosorbent assays (ELISA) for biomarker detection.
  • Enables robust coupling of antigens or antibodies, enhancing assay sensitivity.
• Biosensor Development:
  • Functionalization of biosensors for detecting specific analytes, such as small molecules or proteins.
  • Commonly used in optical, electrochemical, or piezoelectric biosensor platforms.
• Drug Discovery:
  • High-throughput screening of compounds interacting with immobilized targets.
  • Enables studies of ligand-receptor interactions under stable conditions.

Carboxylated Plates are indispensable in applications requiring robust and stable biomolecule attachment. Their versatility, chemical stability, and compatibility with high-throughput systems make them a valuable tool in molecular biology, diagnostics, and drug development.