Carboxylated Plates

Carboxylated Plates are microplates coated with carboxyl groups (-COOH), which introduce a negatively charged surface. This coating facilitates the binding of positively charged molecules, such as proteins, peptides, and other biomolecules, through electrostatic interactions. Carboxylated plates are commonly used in various biochemical and molecular biology applications, including protein immobilization, cell adhesion studies, and assay development.

• Material Composition:
  • Plates are generally made from polystyrene or other durable plastics suitable for biological assays.
  • The surface of the plates is functionalized with carboxyl groups to provide a negatively charged environment.
• Carboxylation Process:
  • Carboxylated plates are created through a chemical modification process where carboxyl groups are introduced onto the plate surface.
  • This process ensures uniform distribution of carboxyl groups across the wells, enhancing the binding capacity for positively charged molecules.
• Binding Properties:
  • The negatively charged carboxyl groups interact electrostatically with positively charged molecules, facilitating their adsorption to the plate surface.
  • Carboxyl groups can also participate in covalent bonding reactions with proteins and other biomolecules, depending on the assay conditions.
• Plate Formats:
  • Available in standard formats such as 96-well and 384-well plates, accommodating both low- and high-throughput applications.
  • Typically flat-bottom designs are used, allowing for compatibility with optical detection methods like colorimetric, fluorescent, and chemiluminescent assays.
• Applications:
  • Protein Immobilization: Ideal for capturing and studying positively charged proteins, peptides, and other biomolecules.
  • Cell Adhesion Studies: Facilitates the attachment of positively charged cell surfaces, improving cell culture conditions and assay results.
  • Enzyme Assays: Useful for assays where positively charged enzymes need to be immobilized for activity measurements or interaction studies.
  • Immunoassays: Enhances the binding of antibodies or other proteins in immunoassays, providing a stable platform for various detection methods.
• Compatibility:
  • Compatible with various detection systems, including colorimetric (e.g., HRP/TMB), fluorescent, and luminescent techniques.
  • Suitable for use with standard laboratory equipment, including automated liquid handling systems and microplate readers.
• Well Volume:
  • Designed to handle typical assay volumes, usually ranging from 50 µL to 300 µL per well, depending on the specific plate format and assay requirements.
  • Ensures uniform well-to-well volume, which is critical for obtaining consistent and reproducible results.