ELISA Plates

ELISA Plates are microplates specifically designed for Enzyme-Linked Immunosorbent Assays (ELISA), a widely used technique for detecting and quantifying soluble substances such as proteins, antibodies, hormones, and other biomolecules. These plates are optimized for high-binding capacity and consistency, ensuring reliable assay performance across different wells and experiments.

  • Material Composition:
    • Typically made from polystyrene, a plastic that supports strong protein adsorption, essential for immobilizing antigens, antibodies, or other biomolecules on the plate surface.
    • Available in various surface treatments, such as high-binding, medium-binding, or low-binding, to accommodate different assay requirements.
  • Plate Formats:
    • Common formats include 96-well and 384-well plates, with 96-well plates being the most widely used for standard ELISA assays.
    • Plates are available in flat-bottom, round-bottom, or V-bottom designs, depending on the assay's needs and the type of detection used (colorimetric, fluorescent, or luminescent).
  • Binding Capacity:
    • High-binding plates can adsorb large amounts of proteins, typically 400-500 ng/cm², making them suitable for assays where sensitivity is critical.
    • Medium and low-binding plates are used for applications where excessive binding might lead to high background noise or when working with smaller biomolecules.
  • Surface Treatments:
    • High-binding plates: Treated to maximize protein adsorption, ideal for assays requiring the capture of antibodies, antigens, or other proteins.
    • Streptavidin-coated plates: Designed for assays involving biotinylated molecules, where strong and specific binding to streptavidin is needed.
    • Cell culture-treated plates: Used when live cells are involved in the assay, supporting cell adhesion and growth.
  • Compatibility:
    • Compatible with various detection methods, including colorimetric (e.g., HRP/TMB), fluorescent, and luminescent readouts.
    • Suitable for use with automated plate readers, ensuring consistency and high throughput.
  • Applications:
    • Direct ELISA: Antigens are directly coated on the plate, followed by detection with an enzyme-linked antibody.
    • Indirect ELISA: Primary antibody is detected by an enzyme-linked secondary antibody, often used to quantify antibody levels.
    • Sandwich ELISA: Capture antibody is coated on the plate, which binds to the target antigen, followed by detection with another enzyme-linked antibody, providing high sensitivity.
    • Competitive ELISA: Involves the competition between labeled and unlabeled antigen for binding to the antibody, often used for small molecule detection.
  • Well Volume:
    • Typically designed to hold assay volumes ranging from 50 µL to 300 µL per well, depending on the plate type.
    • Consistent well-to-well volumes ensure uniform reaction conditions across the plate.
AffiPLATE® High Binding 96 well Solid plates, Black
CAT# AFG-BMT-354
Size: 96 Well Plates
512.00 512.0 USD
AffiPLATE® High Binding 96 well Solid plates, White
CAT# AFG-BMT-353
Size: 96 Well Plates
512.00 512.0 USD
AffiPLATE® High Binding 96 well Solid plates, Clear
CAT# AFG-BMT-352
Size: 96 Well Plates
468.80 468.8 USD
512.00 512.0 USD
512.00 512.0 USD
483.20 483.2 USD
512.00 512.0 USD
512.00 512.0 USD
483.20 483.2 USD
AffiPLATE® Medium Binding 96 well Solid plates, Black
CAT# AFG-BMT-345
Size: 96 Well Plates
483.20 483.2 USD
AffiPLATE® Medium Binding 96 well Solid plates, White
CAT# AFG-BMT-344
Size: 96 Well Plates
483.20 483.2 USD
AffiPLATE® Medium Binding 96 well Solid plates, Clear
CAT# AFG-BMT-343
Size: 96 Well Plates
440.00 440.0 USD
483.20 483.2 USD
483.20 483.2 USD
468.80 468.8 USD
483.20 483.2 USD
483.20 483.2 USD
468.80 468.8 USD