Hb Streptavidin Coated Plates

Hb Streptavidin Coated Plates are specialized microplates designed for high-sensitivity assays involving biotinylated molecules. These plates are specifically coated with a high-binding (Hb) version of streptavidin, which enhances the immobilization capacity for biotinylated targets. This feature makes them particularly useful in applications where maximizing the capture of biotinylated molecules is critical, such as in highly sensitive ELISAs, affinity binding assays, and diagnostic testing.

• Material Composition:
  • The plates are generally made from polystyrene, a material known for its durability and suitability for biochemical assays.
  • The surface is coated with a high-binding form of streptavidin, optimized to increase the density of biotin-binding sites on the plate.
• High-Binding Properties:
  • The high-binding (Hb) streptavidin used in these plates enhances the capture efficiency for biotinylated molecules, leading to increased assay sensitivity.
  • Streptavidin's affinity for biotin remains in the femtomolar range, ensuring a stable and specific interaction.
• Surface Coating:
  • The coating process ensures a dense and uniform layer of Hb streptavidin, maximizing the available binding sites and improving the consistency of assay results.
  • Plates may be pre-blocked with an inert protein to minimize non-specific binding, although additional blocking steps can be added depending on the assay.
• Plate Formats:
  • Available in standard 96-well and 384-well formats, making them adaptable for both routine assays and high-throughput screening.
  • Flat-bottom wells are typical, facilitating easy optical detection for a variety of assay readouts, including colorimetric, fluorescent, and chemiluminescent.
• Applications:
  • High-Sensitivity ELISA: Ideal for assays where detecting low concentrations of a biotinylated target is crucial, such as in diagnostic tests or research assays requiring high sensitivity.
  • Molecular Interaction Studies: Suitable for studying interactions between biotinylated ligands and their binding partners in a highly controlled environment.
  • Affinity Purification: Used to selectively capture and purify biotinylated molecules from complex biological samples, benefiting from the enhanced binding capacity.
  • Drug Discovery: Facilitates the screening of biotinylated compounds in drug discovery efforts, particularly in assays requiring high binding capacity and low background.
• Compatibility:
  • Compatible with a wide range of detection systems, including colorimetric, fluorescent, and luminescent techniques, depending on the assay requirements.
  • Designed for use with standard laboratory equipment, including automated liquid handling systems and plate readers.
• Well Volume:
  • Wells are designed to accommodate volumes typically ranging from 50 µL to 300 µL, providing flexibility for different assay protocols.
  • Uniform well dimensions ensure reproducibility and consistent results across the plate.