Heparin Catcher Plates

Heparin Catcher Plates are specialized microplates designed for the selective binding and immobilization of heparin. These plates utilize specific chemical or biochemical surface modifications to capture heparin molecules from biological samples. The technology is particularly valuable in research and clinical applications that involve studying heparin-protein interactions, anticoagulant activities, or drug screening.

Content:

Key Features and Characteristics

• Material Composition:
  • Typically made from polystyrene, glass, or other inert substrates.
  • Coated or functionalized with materials (e.g., polylysine, cationic polymers, or specific ligands) to bind heparin selectively.
• Binding Mechanism:
  • Exploits the highly negative charge of heparin due to its sulfate and carboxylate groups.
  • Positively charged surfaces or heparin-binding proteins/ligands facilitate strong and specific interactions.
• Surface Chemistry:
  • Uniform coating ensures consistent heparin immobilization.
  • Resistant to nonspecific adsorption of other biomolecules.
• Compatibility:
  • Designed for compatibility with standard laboratory equipment like plate readers and automated handling systems.
  • Supports various assay formats, including fluorescence, ELISA, and colorimetric detection.

Applications

• Biochemical Assays:
  • Heparin-Protein Interaction Studies: Investigate the binding of proteins (e.g., antithrombin, growth factors) to immobilized heparin.
  • Screening for Heparin-Binding Drugs: Evaluate candidate drugs targeting heparin interactions in anticoagulation or cancer therapies.
• Anticoagulation Research:
  • Quantification and characterization of heparin activity in blood plasma.
  • Analysis of heparin derivatives or analogs for therapeutic use.
• Diagnostics:
  • Detection of heparin levels in biological samples for monitoring anticoagulant therapies.
  • Development of diagnostic kits for heparin-induced thrombocytopenia (HIT).
• Drug Development:
  • High-throughput screening for compounds that modulate heparin's biological activity.
  • Characterization of heparin analogs with improved pharmacological profiles.

Heparin Catcher Plates play a critical role in research areas like anticoagulation, drug development, and diagnostics, offering a robust and efficient platform for heparin analysis and interaction studies.