Overview of Jacalin Coated Plates

Jacalin Coated Plates, branded as AffiPLATE, are high-quality laboratory tools designed for glycoprotein binding and lectin-based assays. These plates are pre-coated with Jacalin, a lectin that specifically binds to galactose and N-acetylgalactosamine residues, making them highly effective for isolating and analyzing glycoproteins.

Technical Content

Key Features:

• High Binding Specificity: Jacalin exhibits strong affinity towards galactose and N-acetylgalactosamine, allowing for efficient capture of glycoproteins.
• Enhanced Sensitivity: The high binding capacity of Jacalin increases the sensitivity of assays, enabling the detection of low-abundance glycoproteins.
• Versatile Applications: Suitable for various applications including ELISA, Western blot, and glycoprotein purification.
• Pre-coated Convenience: Ready-to-use plates reduce preparation time and minimize variability, ensuring consistent results.

Applications:

• Glycoprotein Analysis: Ideal for studying glycoprotein structure, function, and interactions.
• Lectin-Based Assays: Used in lectin-based ELISA for detecting and quantifying glycoproteins in complex samples.
• Protein Purification: Facilitates the purification of glycoproteins from cell lysates or serum samples.

Usage Protocol:

• Sample Preparation: Prepare the sample by diluting it to the desired concentration.
• Incubation: Add the sample to the Jacalin-coated wells and incubate to allow binding.
• Washing: Wash the wells to remove unbound materials, ensuring that only glycoproteins remain bound.
• Detection: Use appropriate detection reagents (e.g., secondary antibodies, enzyme substrates) to visualize and quantify the bound glycoproteins.
• Analysis: Analyze the results using a plate reader or imaging system.

Benefits:

• Time Efficiency: Pre-coated plates streamline the workflow, saving valuable research time.
• Reproducibility: Consistent coating ensures reproducibility across experiments.
• Cost-Effective: Reduces the need for additional reagents and materials.