Jacalin Coated Plates

Jacalin-coated plates are specialized microplates coated with Jacalin, a plant lectin extracted from Artocarpus integrifolia (jackfruit seeds). Jacalin has high specificity for galactose and N-acetylgalactosamine (GalNAc), making these plates ideal for studying glycoproteins, mucins, and other galactose-containing biomolecules. They are widely used in glycoprotein analysis, diagnostics, and carbohydrate research.

Content and Properties of Jacalin-Coated Plates

• Material Composition:
  • Plates are typically made from high-quality polystyrene, coated with Jacalin via adsorption or covalent methods.
  • The coating ensures strong and stable binding of galactose-containing biomolecules.
• Binding Specificity:
  • Binds glycoproteins with O-linked glycosylation, particularly those containing GalNAc residues.
  • High affinity for mucins and other heavily glycosylated proteins.
• Well Formats:
  • Available in standard 96-well and 384-well formats for flexibility in assay scale.
  • Flat-bottom designs ensure compatibility with most detection instruments.
• Surface Variants:
  • High-binding surfaces enhance the immobilization of glycoproteins.
  • Low background interference supports sensitive detection.

Applications of Jacalin-Coated Plates

• Glycoprotein Binding and Detection:
  • Used to capture and analyze glycoproteins, such as mucins, IgA, or other O-glycosylated proteins.
• Carbohydrate Interaction Studies:
  • Suitable for studying interactions between galactose-containing carbohydrates and proteins.
• Enzyme-Linked Lectin Assays (ELLA):
  • Jacalin serves as a lectin probe in lectin-based immunoassays for detecting specific carbohydrate structures.
• Diagnostics:
  • Detection of glycosylation patterns associated with diseases, such as cancer or autoimmune disorders.
• Mucin Analysis:
  • Commonly used to study mucins and their glycosylation profiles in biological samples.

Jacalin-coated plates are powerful tools for glycoprotein research, diagnostics, and carbohydrate-protein interaction studies. Their high specificity for GalNAc-containing biomolecules makes them particularly valuable in mucin analysis, immune studies, and disease biomarker detection.