Overview of Maleimide Coated Plates

Maleimide Coated Plates are specialized laboratory tools designed for the covalent immobilization of sulfhydryl-containing biomolecules. These plates feature a surface coated with maleimide groups, which react specifically with thiol groups, providing a stable and efficient method for biomolecule attachment.

Technical Content

Key Features:

• High Reactivity: Maleimide groups on the plate surface react specifically with thiol groups, forming stable thioether bonds.
• Specificity: High specificity for sulfhydryl-containing biomolecules, minimizing non-specific binding.
• Versatile Applications: Suitable for immobilizing a wide range of biomolecules, including peptides, proteins, and antibodies.

Applications:

• Protein-Protein Interaction Studies: Used to immobilize thiol-containing proteins for studying protein interactions.
• Antibody Immobilization: Facilitates the stable attachment of antibodies for ELISA and other immunoassays.
• Biosensor Development: Assists in creating biosensors by immobilizing thiol-modified biomolecules on sensor surfaces.

Usage Protocol:

• Preparation: Prepare the sulfhydryl-containing biomolecule solution according to experimental requirements.
• Coating: Add the biomolecule solution to the wells of the Maleimide Coated Plate.
• Incubation: Incubate the plate to allow the maleimide groups to react with the thiol groups, forming covalent bonds.
• Washing: Wash the wells to remove unbound biomolecules, ensuring that only covalently attached molecules remain.
• Application: Use the coated plate for downstream applications, such as ELISA, biosensing, or interaction studies.

Benefits:

• Stability: Covalent attachment ensures that biomolecules remain firmly attached under various experimental conditions.
• Efficiency: Ready-to-use plates simplify the immobilization process, reducing preparation time and labor.
• Reproducibility: Provides consistent and reproducible results across experiments due to stable covalent binding.