Maleimide Coated Plates

Maleimide Coated Plates are specialized microplates functionalized with maleimide groups (-C=CH-CH=CH2), which provide a highly specific surface for covalent attachment of thiol (-SH) groups. These plates are widely used in applications requiring stable and oriented immobilization of sulfhydryl-containing biomolecules, such as proteins, peptides, or small molecules, making them ideal for bioassays, diagnostics, and biosensor development.

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Key Features and Characteristics

• Surface Chemistry:
  • Maleimide groups on the surface enable covalent bonding through Michael addition with thiol groups.
  • Forms stable thioether bonds, ensuring long-term immobilization.
• Specificity:
  • Reacts selectively with sulfhydryl groups, minimizing nonspecific binding.
  • Maintains biomolecule orientation, preserving activity and functionality.
• Material Composition:
  • Plates are typically made from polystyrene or glass.
  • Functionalized with maleimide groups using chemical methods, ensuring uniform coating.

Applications

• Protein and Peptide Immobilization:
  • Covalent attachment of thiol-containing proteins (e.g., enzymes, antibodies) for use in ELISA, biosensors, and other bioassays.
  • Immobilization of cysteine-modified peptides for epitope mapping and interaction studies.
• Biosensor Development:
  • Functionalization of biosensors with thiol-modified molecules for specific analyte detection.
  • Commonly used in electrochemical and optical sensor platforms.
• Drug Discovery:
  • High-throughput screening of compounds interacting with immobilized targets.
  • Suitable for studying enzyme-inhibitor interactions or ligand binding.

Maleimide Coated Plates are essential for applications where specific, stable, and functional immobilization of thiol-containing biomolecules is required. Their precise chemistry and compatibility with diverse biomolecules make them invaluable tools in molecular biology, diagnostics, and nanotechnology.