Neutravidin Coated Plates

NeutrAvidin Coated Plates are microplates designed for assays involving the binding of biotinylated molecules. NeutrAvidin, a deglycosylated form of avidin, is used as the coating protein because it retains high affinity for biotin while minimizing non-specific binding and background interference. These plates are particularly suited for applications requiring reduced non-specific interactions, such as in sensitive ELISAs, affinity binding assays, and other biotin-streptavidin/avidin based protocols.

• Material Composition:
  • Plates are typically made from polystyrene, a durable plastic that is well-suited for various biochemical assays.
  • The surface is coated with NeutrAvidin, which is a modified version of avidin that has been deglycosylated to reduce non-specific binding.
• Binding Properties:
  • NeutrAvidin binds biotin with high affinity, similar to streptavidin, with a dissociation constant (Kd) in the femtomolar range.
  • Unlike avidin, NeutrAvidin is non-glycosylated, reducing non-specific binding and background signal in assays, making it ideal for high-precision work.
• Surface Coating:
  • The coating process ensures uniform distribution of NeutrAvidin across the well surface, optimizing the number of available binding sites for biotinylated molecules.
  • Plates are typically pre-blocked to minimize non-specific interactions, although additional blocking steps may be applied depending on the assay.
• Plate Formats:
  • Available in standard 96-well and 384-well formats, accommodating both low- and high-throughput assay requirements.
  • Plates come in flat-bottom configurations to facilitate easy optical detection, including colorimetric, fluorescent, and chemiluminescent readouts.
• Applications:
  • ELISA: Used in assays where biotinylated antibodies or antigens are captured and detected, offering high sensitivity with low background interference.
  • Molecular Interaction Studies: Facilitates precise studies of biotinylated ligands and their binding partners with minimal non-specific interactions.
  • Affinity Purification: Enables the selective capture and isolation of biotinylated proteins, peptides, or nucleic acids from complex mixtures with reduced non-specific binding.
  • Diagnostic Assays: Suitable for diagnostic tests requiring high sensitivity and specificity, particularly in clinical settings where sample clarity is essential.
• Compatibility:
  • Compatible with a variety of detection methods, including colorimetric (e.g., HRP/TMB), fluorescent, and luminescent systems.
  • Suitable for use with standard laboratory equipment such as automated liquid handlers and microplate readers, ensuring broad compatibility with existing workflows.
• Well Volume:
  • Typically designed to hold reaction volumes ranging from 50 µL to 300 µL per well, depending on the specific assay format.
  • Consistent well dimensions across the plate ensure uniform sample handling and reaction conditions.