Neutravidin Coated Plates

NeutrAvidin-coated plates are microplates designed for the immobilization of biotinylated molecules, offering an alternative to streptavidin-coated plates. NeutrAvidin, a deglycosylated version of avidin, retains high biotin-binding affinity while reducing nonspecific binding due to its neutral charge. These plates are widely used in molecular biology, immunology, diagnostics, and high-throughput screening applications.

Content and Properties of NeutrAvidin-Coated Plates

• Material Composition:
  • Plates are typically made of high-quality polystyrene, treated for optimal protein adsorption and coated with NeutrAvidin.
  • NeutrAvidin is covalently or adsorptively immobilized for stable and consistent binding.
• Binding Characteristics:
  • High-affinity binding to biotin, with minimal nonspecific interactions.
  • Suitable for immobilizing biotinylated proteins, peptides, nucleic acids, or small molecules.
• Well Formats and Surface Variants:
  • Available in 96-well, 384-well, and other formats for different throughput needs.
  • Offered in transparent, black, or white variants for colorimetric, fluorescence, or luminescence detection.
• Compatibility:
  • Designed for use with biotinylated molecules in manual or automated systems.
  • Works seamlessly with standard detection systems and instrumentation.

Applications of NeutrAvidin-Coated Plates

• Biotinylated Biomolecule Immobilization:
  • Suitable for binding biotinylated antibodies, antigens, oligonucleotides, or peptides in ELISA and other assays.
• Enzyme-Linked Immunosorbent Assays (ELISA):
  • Commonly used in sandwich or competitive ELISA formats for antigen or antibody detection.
• Nucleic Acid Assays:
  • Applied in DNA/RNA hybridization assays with biotinylated probes.
• High-Throughput Screening:
  • Ideal for drug discovery and biomolecular interaction studies.
• Diagnostics:
  • Used in clinical assays for detecting biomarkers, infectious agents, or genetic variations.

NeutrAvidin-coated plates provide a reliable and low-background platform for assays requiring biotinylated molecule immobilization. Their reduced nonspecific binding makes them particularly valuable in sensitive applications where precision and reproducibility are critical.