Neutravidin Coated Plates
NeutrAvidin-coated plates are microplates designed for the immobilization of biotinylated molecules, offering an alternative to streptavidin-coated plates. NeutrAvidin, a deglycosylated version of avidin, retains high biotin-binding affinity while reducing nonspecific binding due to its neutral charge. These plates are widely used in molecular biology, immunology, diagnostics, and high-throughput screening applications.
Content and Properties of NeutrAvidin-Coated Plates
- Material Composition:
- Plates are typically made of high-quality polystyrene, treated for optimal protein adsorption and coated with NeutrAvidin.
- NeutrAvidin is covalently or adsorptively immobilized for stable and consistent binding.
- Binding Characteristics:
- High-affinity binding to biotin, with minimal nonspecific interactions.
- Suitable for immobilizing biotinylated proteins, peptides, nucleic acids, or small molecules.
- Well Formats and Surface Variants:
- Available in 96-well, 384-well, and other formats for different throughput needs.
- Offered in transparent, black, or white variants for colorimetric, fluorescence, or luminescence detection.
- Compatibility:
- Designed for use with biotinylated molecules in manual or automated systems.
- Works seamlessly with standard detection systems and instrumentation.
Applications of NeutrAvidin-Coated Plates
- Biotinylated Biomolecule Immobilization:
- Suitable for binding biotinylated antibodies, antigens, oligonucleotides, or peptides in ELISA and other assays.
- Enzyme-Linked Immunosorbent Assays (ELISA):
- Commonly used in sandwich or competitive ELISA formats for antigen or antibody detection.
- Nucleic Acid Assays:
- Applied in DNA/RNA hybridization assays with biotinylated probes.
- High-Throughput Screening:
- Ideal for drug discovery and biomolecular interaction studies.
- Diagnostics:
- Used in clinical assays for detecting biomarkers, infectious agents, or genetic variations.
NeutrAvidin-coated plates provide a reliable and low-background platform for assays requiring biotinylated molecule immobilization. Their reduced nonspecific binding makes them particularly valuable in sensitive applications where precision and reproducibility are critical.
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