No Binding Capacity Plates

No Binding Capacity Plates are specially designed microplates with surfaces engineered to minimize or eliminate nonspecific binding of biomolecules. These plates are typically treated with hydrophilic coatings or inert substances, such as silanes or polymers like polyethylene glycol (PEG), to reduce adsorption of proteins, nucleic acids, or other macromolecules.

Content: 

Key Features and Characteristics

• Surface Chemistry:
  • Treated with hydrophilic or inert coatings that resist adsorption of biomolecules.
  • Typically uses polymers like PEG or silane-based treatments to create a non-reactive surface.
• Material:
  • Plates are generally made of polystyrene, polypropylene, or glass, with a specialized surface treatment.
  • Available in opaque, clear, or black formats to suit different detection methods.
• Binding Resistance:
  • Designed to eliminate or minimize protein, DNA/RNA, and small molecule binding.
  • Reduces nonspecific interactions, preserving assay specificity.
• Compatibility:
  • Suitable for fluorescence, chemiluminescence, and absorbance-based detection systems.
  • Often used in applications requiring high sensitivity and low background noise.

Applications

• Low-Abundance Target Detection:
  • Ideal for detecting low concentrations of analytes without interference from nonspecific binding.
  • Commonly used in assays involving rare biomarkers or low-input samples.
• Cell-Based Assays:
  • Supports suspension cell cultures where cell attachment is not required.
  • Prevents cell adhesion, useful for spheroid or 3D culture models.
• Biomolecular Assays:
  • Minimizes nonspecific binding in sensitive assays such as ligand-binding studies or receptor-ligand interaction experiments.
  • Reduces background in ELISA, protein-protein interaction studies, or nucleic acid hybridization assays.

No Binding Capacity Plates are essential tools for applications where nonspecific adsorption can compromise sensitivity and specificity. Their ability to reduce background noise makes them particularly valuable in diagnostics, drug discovery, and advanced molecular biology research.