Poly Arginine Coated Plates
Poly Arginine Coated Plates are specialized microplates coated with poly-L-arginine, a positively charged polymer. These plates are primarily used to enhance the attachment of negatively charged molecules, such as nucleic acids (DNA/RNA), proteins, and certain types of cells, to the plate surface. They are widely employed in applications like cell culture, nucleic acid immobilization, and various biochemical assays requiring stable substrate adhesion.
- Material Composition:
- The plates are generally made from polystyrene, a material known for its robustness and compatibility with biological assays.
- The surface is coated with poly-L-arginine, a synthetic polymer composed of multiple arginine residues, giving it a strong positive charge.
- Poly Arginine Coating:
- Poly-L-arginine provides a highly positively charged surface that facilitates the electrostatic binding of negatively charged biomolecules.
- The coating is uniform across the plate surface, ensuring consistent binding properties across all wells.
- Binding Properties:
- The positive charge of poly-L-arginine strongly attracts and binds negatively charged molecules like nucleic acids and proteins, promoting stable attachment.
- The coating enhances cell adhesion, particularly for cells that naturally have a negative surface charge, improving cell culture and assay outcomes.
- Plate Formats:
- Available in standard formats, such as 96-well and 384-well plates, catering to both low and high-throughput experimental needs.
- Typically flat-bottom plates are used, which are compatible with optical detection methods in various assays.
- Applications:
- Nucleic Acid Immobilization: Used to immobilize DNA, RNA, or other nucleic acids onto the plate surface for hybridization assays, PCR, or other molecular biology techniques.
- Cell Culture: Enhances the attachment of cells, particularly those with weak adherence properties, to the plate surface, which is useful in various cell-based assays.
- Protein Binding Assays: Facilitates the immobilization of negatively charged proteins for studies involving protein-protein interactions or enzyme assays.
- Transfection Studies: Can be used to improve the efficiency of cell transfection by enhancing the adherence of nucleic acids to cells during the transfection process.
- Compatibility:
- Compatible with various detection methods, including colorimetric, fluorescent, and luminescent assays, depending on the application.
- Suitable for use with standard laboratory equipment, including automated pipetting systems and microplate readers, ensuring integration into existing workflows.
- Well Volume:
- Designed to accommodate typical assay volumes, usually ranging from 50 µL to 300 µL per well, depending on the specific format and experimental setup.
- Ensures consistent well-to-well volume, which is essential for reproducibility in high-throughput experiments.
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