Overview of Poly-L-Lysine Coated Plates

Poly-L-Lysine Coated Plates are specialized laboratory tools designed to enhance the adhesion of cells and biomolecules to the surface of the plate. These plates are coated with Poly-L-Lysine, a synthetic amino acid polymer that promotes strong electrostatic interactions between the plate surface and negatively charged molecules or cell membranes.

Technical Content

Key Features:

• Enhanced Cell Adhesion: The Poly-L-Lysine coating significantly improves the attachment and spreading of cells on the plate surface.
• High Compatibility: Suitable for a wide range of cell types, including primary cells, cell lines, and neurons.
• Improved Biomolecule Binding: Facilitates the immobilization of proteins, nucleic acids, and other biomolecules for various assays.

Applications:

• Cell Culture: Used to culture cells that are difficult to adhere to standard plastic or glass surfaces.
• Microscopy: Ideal for preparing samples for fluorescence or electron microscopy by ensuring cells remain attached during washing steps.
• Assay Development: Enhances the performance of various biochemical and immunological assays by improving biomolecule binding to the plate surface.

Usage Protocol:

• Preparation: Ensure the Poly-L-Lysine Coated Plate is at room temperature before use.
• Cell Seeding: Seed cells onto the plate at the desired density and allow them to adhere under appropriate culture conditions.
• Incubation: Incubate the plate to allow for optimal cell attachment and spreading.
• Assay Performance: Proceed with downstream applications, such as staining, imaging, or biochemical assays, ensuring minimal disturbance to the adherent cells.

Benefits:

• Stability: Provides a stable surface for cell adhesion, reducing cell loss during washing and handling.
• Versatility: Compatible with a variety of cell types and biomolecules, making it suitable for diverse research applications.
• Efficiency: Ready-to-use plates simplify experimental setup and reduce preparation time.