Poly-L-Lysine Coated Plates
Poly-L-Lysine Coated Plates are specialized laboratory tools designed to enhance the adhesion of cells and biomolecules to the surface of the plate. These plates are coated with Poly-L-Lysine, a synthetic amino acid polymer that promotes strong electrostatic interactions between the plate surface and negatively charged molecules or cell membranes.
Key Features:
- Enhanced Cell Adhesion: The Poly-L-Lysine coating significantly improves the attachment and spreading of cells on the plate surface.
- High Compatibility: Suitable for a wide range of cell types, including primary cells, cell lines, and neurons.
- Improved Biomolecule Binding: Facilitates the immobilization of proteins, nucleic acids, and other biomolecules for various assays.
Applications:
- Cell Culture: Used to culture cells that are difficult to adhere to standard plastic or glass surfaces.
- Microscopy: Ideal for preparing samples for fluorescence or electron microscopy by ensuring cells remain attached during washing steps.
- Assay Development: Enhances the performance of various biochemical and immunological assays by improving biomolecule binding to the plate surface.
Usage Protocol:
- Preparation: Ensure the Poly-L-Lysine Coated Plate is at room temperature before use.
- Cell Seeding: Seed cells onto the plate at the desired density and allow them to adhere under appropriate culture conditions.
- Incubation: Incubate the plate to allow for optimal cell attachment and spreading.
- Assay Performance: Proceed with downstream applications, such as staining, imaging, or biochemical assays, ensuring minimal disturbance to the adherent cells.
Benefits:
- Stability: Provides a stable surface for cell adhesion, reducing cell loss during washing and handling.
- Versatility: Compatible with a variety of cell types and biomolecules, making it suitable for diverse research applications.
- Efficiency: Ready-to-use plates simplify experimental setup and reduce preparation time.
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