Poly-L-Lysine Coated Plates

Poly-L-lysine-coated plates are specialized microplates pre-coated with poly-L-lysine, a synthetic positively charged polymer. These plates are commonly used to enhance cell adhesion, immobilize biomolecules, or facilitate specific experimental setups in molecular biology, cell culture, and immunology. The positively charged surface interacts strongly with negatively charged molecules, such as cell membranes or nucleic acids.

Content and Properties of Poly-L-Lysine-Coated Plates

• Material Composition:
  • Plates are made from polystyrene or similar materials and uniformly coated with poly-L-lysine.
  • The poly-L-lysine layer enhances surface charge, promoting strong interactions with negatively charged biomolecules.
• Surface Properties:
  • Highly hydrophilic surface with strong electrostatic interactions.
  • Suitable for immobilizing cells, proteins, or nucleic acids.
• Well Formats:
  • Available in 96-well, 384-well, and other configurations to accommodate different experimental scales.
  • Flat-bottom and round-bottom designs are common for various assay requirements.
• Compatibility:
  • Works with diverse cell types, especially adherent cells, to improve attachment and spreading.
  • Supports immobilization of biomolecules like DNA, RNA, and proteins for hybridization or binding assays.

Applications of Poly-L-Lysine-Coated Plates

• Cell Culture:
  • Enhances adhesion and spreading of adherent cell lines, primary cells, or neurons.
  • Used for culturing difficult-to-adhere cells.
• Immunocytochemistry (ICC) and Immunofluorescence:
  • Provides a stable surface for cell fixation and antibody staining.
• Hybridization Assays:
  • Facilitates immobilization of nucleic acids, such as DNA or RNA, for hybridization-based experiments.
• Protein Immobilization:
  • Allows attachment of negatively charged proteins or peptides for ELISA or binding assays.
• Microscopy:
  • Used for preparing slides or plates for imaging experiments, ensuring stable cell or tissue adhesion.

Poly-L-lysine-coated plates are valuable tools for enhancing adhesion and immobilization in a wide range of biological and diagnostic applications. Their electrostatic properties make them particularly useful for assays requiring stable attachment of cells or biomolecules, ensuring reliable and reproducible results.