Protein Coated Plates
Protein Coated Plates are microplates specifically designed for assays that require the immobilization of proteins on the surface. These plates are used in a variety of biochemical and cell biology applications, including enzyme-linked immunosorbent assays (ELISA), protein-protein interaction studies, and cell adhesion assays. The plates are coated with specific proteins or protein-binding molecules, allowing for the stable and consistent attachment of target molecules or cells to the plate surface.
- Material Composition:
- Typically made from polystyrene, known for its compatibility with biological assays and its ability to adsorb proteins effectively.
- Plates may be treated to enhance protein binding, ensuring strong and uniform attachment of the coated protein to the surface.
- Protein Coating:
- Plates can be coated with a variety of proteins, such as antibodies, antigens, enzymes, or other specific binding proteins.
- Coating is usually performed under controlled conditions to ensure that the protein is uniformly distributed and retains its biological activity.
- Common coatings include BSA (bovine serum albumin), collagen, fibronectin, or custom proteins depending on the intended application.
- Surface Characteristics:
- High protein-binding capacity ensures that the immobilized protein is stable and remains functional throughout the assay.
- Surface treatments can be tailored to the specific needs of the assay, such as hydrophobic or hydrophilic coatings, depending on the protein being used.
- Plate Formats:
- Available in standard formats, such as 96-well and 384-well plates, suitable for low to high-throughput applications.
- Plates may be flat-bottom, U-bottom, or V-bottom, depending on the type of assay and the method of detection used.
- Applications:
- ELISA: Used for capturing and detecting specific proteins, antibodies, or antigens, with the coated protein serving as the capture or detection molecule.
- Protein-Protein Interaction Studies: Allows for the investigation of interactions between immobilized proteins and their binding partners in solution.
- Cell Adhesion Assays: Coated with extracellular matrix proteins (e.g., collagen, fibronectin) to study cell adhesion, migration, or morphology in a controlled environment.
- Receptor-Ligand Binding Assays: Used to study interactions between immobilized receptors and their ligands.
- Compatibility:
- Compatible with various detection methods, including colorimetric, fluorescent, and luminescent assays, depending on the experimental setup.
- Suitable for use with standard laboratory equipment, including pipettes, automated liquid handlers, and microplate readers.
- Well Volume:
- Designed to accommodate typical assay volumes, usually ranging from 50 µL to 300 µL per well, ensuring consistent reaction conditions across the plate.
- Well-to-well uniformity is critical for reproducible results, especially in high-throughput assays.
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