Protein Coated Plates

Protein Coated Plates are microplates specifically designed for assays that require the immobilization of proteins on the surface. These plates are used in a variety of biochemical and cell biology applications, including enzyme-linked immunosorbent assays (ELISA), protein-protein interaction studies, and cell adhesion assays. The plates are coated with specific proteins or protein-binding molecules, allowing for the stable and consistent attachment of target molecules or cells to the plate surface.

  • Material Composition:
    • Typically made from polystyrene, known for its compatibility with biological assays and its ability to adsorb proteins effectively.
    • Plates may be treated to enhance protein binding, ensuring strong and uniform attachment of the coated protein to the surface.
  • Protein Coating:
    • Plates can be coated with a variety of proteins, such as antibodies, antigens, enzymes, or other specific binding proteins.
    • Coating is usually performed under controlled conditions to ensure that the protein is uniformly distributed and retains its biological activity.
    • Common coatings include BSA (bovine serum albumin), collagen, fibronectin, or custom proteins depending on the intended application.
  • Surface Characteristics:
    • High protein-binding capacity ensures that the immobilized protein is stable and remains functional throughout the assay.
    • Surface treatments can be tailored to the specific needs of the assay, such as hydrophobic or hydrophilic coatings, depending on the protein being used.
  • Plate Formats:
    • Available in standard formats, such as 96-well and 384-well plates, suitable for low to high-throughput applications.
    • Plates may be flat-bottom, U-bottom, or V-bottom, depending on the type of assay and the method of detection used.
  • Applications:
    • ELISA: Used for capturing and detecting specific proteins, antibodies, or antigens, with the coated protein serving as the capture or detection molecule.
    • Protein-Protein Interaction Studies: Allows for the investigation of interactions between immobilized proteins and their binding partners in solution.
    • Cell Adhesion Assays: Coated with extracellular matrix proteins (e.g., collagen, fibronectin) to study cell adhesion, migration, or morphology in a controlled environment.
    • Receptor-Ligand Binding Assays: Used to study interactions between immobilized receptors and their ligands.
  • Compatibility:
    • Compatible with various detection methods, including colorimetric, fluorescent, and luminescent assays, depending on the experimental setup.
    • Suitable for use with standard laboratory equipment, including pipettes, automated liquid handlers, and microplate readers.
  • Well Volume:
    • Designed to accommodate typical assay volumes, usually ranging from 50 µL to 300 µL per well, ensuring consistent reaction conditions across the plate.
    • Well-to-well uniformity is critical for reproducible results, especially in high-throughput assays.

5,192.00 5192.0 USD
5,192.00 5192.0 USD
5,177.60 5177.6 USD
3,852.80 3852.8 USD
3,852.80 3852.8 USD
3,795.20 3795.2000000000003 USD
5,192.00 5192.0 USD
5,192.00 5192.0 USD
5,177.60 5177.6 USD
3,852.80 3852.8 USD
3,852.80 3852.8 USD
3,795.20 3795.2000000000003 USD
5,192.00 5192.0 USD
5,192.00 5192.0 USD
5,177.60 5177.6 USD
3,852.80 3852.8 USD
3,852.80 3852.8 USD
3,795.20 3795.2000000000003 USD
3,118.40 3118.4 USD
3,118.40 3118.4 USD
3,075.20 3075.2000000000003 USD
2,312.00 2312.0 USD
2,312.00 2312.0 USD
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3,118.40 3118.4 USD
3,118.40 3118.4 USD
3,089.60 3089.6 USD
2,312.00 2312.0 USD
2,312.00 2312.0 USD
2,283.20 2283.2000000000003 USD
3,118.40 3118.4 USD
3,118.40 3118.4 USD
3,089.60 3089.6 USD
2,312.00 2312.0 USD
2,312.00 2312.0 USD
2,283.20 2283.2000000000003 USD
3,118.40 3118.4 USD
3,118.40 3118.4 USD
3,075.20 3075.2000000000003 USD
2,312.00 2312.0 USD
2,312.00 2312.0 USD
2,268.80 2268.8 USD
3,118.40 3118.4 USD
3,118.40 3118.4 USD
3,089.60 3089.6 USD
2,312.00 2312.0 USD
2,312.00 2312.0 USD
2,283.20 2283.2000000000003 USD
3,118.40 3118.4 USD
3,118.40 3118.4 USD
3,089.60 3089.6 USD
2,312.00 2312.0 USD
2,312.00 2312.0 USD
2,283.20 2283.2000000000003 USD