Protein Coated Plates
Protein-coated plates are specialized microplates pre-coated with specific proteins, such as antibodies, antigens, enzymes, or other biomolecules, to facilitate binding interactions in biological assays. These plates provide a ready-to-use platform for applications in molecular biology, immunology, diagnostics, and drug discovery, ensuring high specificity and reproducibility.
Content and Properties of Protein-Coated Plates
- Material Composition:
- Made of high-quality polystyrene, optimized for uniform protein adsorption.
- Plates may include surface treatments to enhance binding and stability of immobilized proteins.
- Types of Protein Coatings:
- Antibody-Coated Plates: Pre-coated with capture antibodies for sandwich ELISA and other immunoassays.
- Antigen-Coated Plates: Coated with specific antigens for detecting antibodies in serological tests.
- Enzyme-Coated Plates: Pre-coated with enzymes like horseradish peroxidase (HRP) for substrate conversion studies.
- Custom-Coated Plates: Coated with user-specified proteins for specialized applications.
- Surface Types:
- High-binding surfaces for proteins requiring strong adsorption.
- Low-binding surfaces for applications needing reduced non-specific interactions.
Applications of Protein-Coated Plates
- Enzyme-Linked Immunosorbent Assays (ELISA):
- Widely used in sandwich, competitive, or direct ELISA formats for detecting specific antigens or antibodies.
- Antibody Screening:
- Used in hybridoma screening or monoclonal antibody production.
- Serological Testing:
- Detection of antibodies against infectious agents or biomarkers in clinical diagnostics.
- Drug Discovery:
- High-throughput screening for protein-ligand interactions and pharmacological studies.
- Receptor-Ligand Binding Studies:
- Characterization of protein-protein or protein-small molecule interactions.
Protein-coated plates are versatile and reliable tools in biological research and diagnostics. Their high specificity and ease of use make them essential for immunoassays, interaction studies, and clinical applications where precision and reproducibility are critical.
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