Streptavidin Coated Plates

Streptavidin-coated plates are specialized microplates pre-coated with streptavidin, a protein with high affinity for biotin. These plates are widely used in molecular biology, immunology, and diagnostic applications for immobilizing biotinylated molecules. The strong and specific streptavidin-biotin interaction provides a versatile platform for assays requiring biomolecule immobilization, signal detection, or amplification.

Content and Properties of Streptavidin-Coated Plates

• Material Composition:
  • Plates are made of high-quality polystyrene, chosen for its chemical stability and suitability for surface coating.
  • Streptavidin is immobilized via covalent or adsorption-based methods for long-term stability.
• Binding Capacity:
  • High streptavidin density ensures efficient capture of biotinylated molecules.
  • Uniform coating guarantees consistent assay performance.
• Well Formats:
  • Available in 96-well, 384-well, and other configurations for various throughput requirements.
  • Flat-bottom designs are most common, providing compatibility with plate readers.
• Color Variants:
  • Transparent plates for colorimetric detection.
  • White plates for luminescence assays to enhance signal reflection.

Applications of Streptavidin-Coated Plates

• Biomolecule Immobilization:
  • Used to immobilize biotinylated antibodies, antigens, nucleic acids, or peptides for downstream assays.
• Enzyme-Linked Immunosorbent Assays (ELISA):
  • Ideal for sandwich or competitive ELISA formats where biotinylated capture agents are used.
• Nucleic Acid Assays:
  • Employed in hybridization-based assays, including biotinylated probe capture for DNA or RNA detection.
• Signal Amplification:
  • Enables enhanced signal detection in luminescence or fluorescence assays through biotinylated reporter systems.

Streptavidin-coated plates are essential tools in molecular and immunological research. Their robust and specific interaction with biotinylated molecules makes them a cornerstone in a variety of biological assays, from basic research to clinical diagnostics.